Cancer Institute of the Slovak Academy of Sciences, researchers have recently succeeded in extracting from human adipose tissue-derived mesenchymal stem cells out of (mesenchymal stem cells), and through genetic engineering approaches to synthesis, secretion, and destruction of tumor-oriented "suicide genes." Such gene therapy can be offensive to evade current detection techniques, treatment of small tumors to migrate objects.
Project Leader Dr. Cestmir Altaner said that these fat-derived stem cells are expected to be used for cell-based personalized treatment. Everyone more or less some of the excess fat, fat tissue may become a source of cells required for the treatment of cancer.
Mesenchymal stem cells, by updating the cell to repair damaged tissues and organs. The researchers found that in a mixture of solid tumor cancer cell is prepared to base the development of normal cells have mesenchymal stem cells, speculated that the mesenchymal stem cells in tumors will be treated as damaged organs to migrate to the tumor, it can be used to find the original tumors and small metastases. These stem cells have a certain degree of plasticity, which can be organized according to the specialized micro-environment.
Extracted from human adipose tissue-derived mesenchymal stem cells, researchers have begun to search for a treatment of colon cancer compared with standard chemotherapy agent 5-fluorouracil (5-FU) toxicity to normal cells in a smaller way. They will be mesenchymal stem cells in the culture medium in a large number of amplified, and then use a retrovirus vector cytosine deaminase (cytosine deaminase, CD) gene was inserted into the genome of cells, stem cells, the gene will be a poison fewer side effects the drug 5-fluorocytosine (5-FC) into 5-FU. Then, the researchers used chemotherapy to kill cancer, resulting in a fatal bystander effect (by-stander effect, refers to the killing of tumor cells missiles, targeting tumor cells in the concentration camps, give anti-personnel, but also can kill a tumor adjacent areas of tumor cells).
In the transplantation of human colon cancer in nude mice (no immunity), the researchers genetically engineered to be the first injection of mesenchymal stem cells, then injected 5-FC. The results showed that tumor growth inhibition rate reached 68.5%, no toxic side effects occurred in mice 1. The researchers said that although there is no one mice were completely cured, but our program is very effective, even only once stem cells. Obviously, repeated treatments with better results.
Common mesenchymal stem cells have a variety of sources (such as bone marrow), but the original production did not get more than fat tissue. The easiest way is to remove the tumor from surgery to get fat tissue, liposuction method can also be used to separate mesenchymal stem cells, these two processes to collect than bone marrow from the patient much easier. (Cancer Institute of the Slovak Academy of Sciences, researchers recently succeeded in extracting from human adipose tissue-derived mesenchymal stem cells out of (mesenchymal stem cells), and through genetic engineering approaches to synthesis, secretion, and destruction of tumor-oriented "suicide genes." This is kinds of gene therapy can be offensive to evade the current detection techniques, treatment of small tumors to migrate objects. the details published in the July 1 "Cancer Research" journal.
Project Leader Dr. Cestmir Altaner said that these fat-derived stem cells are expected to be used for cell-based personalized treatment. Everyone more or less some of the excess fat, fat tissue may become a source of cells required for the treatment of cancer.
Mesenchymal stem cells, by updating the cell to repair damaged tissues and organs. The researchers found that in a mixture of solid tumor cancer cell is prepared to base the development of normal cells have mesenchymal stem cells, speculated that the mesenchymal stem cells in tumors will be treated as damaged organs to migrate to the tumor, it can be used to find the original tumors and small metastases. These stem cells have a certain degree of plasticity, which can be organized according to the specialized micro-environment.
Extracted from human adipose tissue-derived mesenchymal stem cells, researchers have begun to search for a treatment of colon cancer compared with standard chemotherapy agent 5-fluorouracil (5-FU) toxicity to normal cells in a smaller way. They will be mesenchymal stem cells in the culture medium in a large number of amplified, and then use a retrovirus vector cytosine deaminase (cytosine deaminase, CD) gene was inserted into the genome of cells, stem cells, the gene will be a poison fewer side effects the drug 5-fluorocytosine (5-FC) into 5-FU. Then, the researchers used chemotherapy to kill cancer, resulting in a fatal bystander effect (by-stander effect, refers to the killing of tumor cells missiles, targeting tumor cells in the concentration camps, give anti-personnel, but also can kill a tumor adjacent areas of tumor cells).
In the transplantation of human colon cancer in nude mice (no immunity), the researchers genetically engineered to be the first injection of mesenchymal stem cells, then injected 5-FC. The results showed that tumor growth inhibition rate reached 68.5%, no toxic side effects occurred in mice 1. The researchers said that although there is no one mice were completely cured, but our program is very effective, even only once stem cells. Obviously, repeated treatments with better results.
Common mesenchymal stem cells have a variety of sources (such as bone marrow), but the original production did not get more than fat tissue. The easiest way is to remove the tumor from surgery to get fat tissue, liposuction method can also be used to separate mesenchymal stem cells, these two processes to collect than bone marrow from the patient much easier.
